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method for hochaufreinigung of human serum albumin
method for hochaufreinigung of human serum albumin
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机译:人血清白蛋白检测方法
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摘要
A method of highly purifying human serum albumin (HSA) which comprises bringing a fraction containing HSA produced by gene manipulation into contact with a chelate chromatography carrier having copper ions combined therewith and eluting the HSA adsorbed on the carrier by using a buffer having a pH of 5 to 7 and containing ammonium chloride as an antagonist. This method can provide a highly pure HSA free from yeast-origin components which have been dificult to remove satisfactorily by the conventional method of purifying HSA produced by gene manipulation.
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