Equipment for automatic analysis and test method for detection of the protein function of the cell surface and / or cytoplasmic receptor using the same.

摘要

PRESENTED AN AUTOMATIC MEASURING APPARATUS AND HIGH EFFICIENCY METHOD FOR PERFORMING PROCESSES AUTOMATICOS drug screening. The apparatus is capable of initiating and MEDIAR INCIDENTS RAPIDOS or transient receptor activity such as AND / OR CELL ion channel. THE APPARATUS invention is capable of predetermined positions aligned in one or more of Samples in container multi-cavity start the reaction with the addition of a reagent and ATTRIBUTE MEASURE RESULTING FOR A PERIOD OF TIME. The apparatus is capable of measuring and recording substantially CONTINUOUS DATA FOR THE ATTRIBUTE measured before, during and after has started REACTION THEREBY DETERMINE THAT MAY SPACE TIME EVENT fast or transient. Once the O REACTIONS IN ONE OR MORE OF THE CAVITY VESSEL multi-cavity, the apparatus can align one or more different CAVITY TO BE TESTED BY THE predetermined position and repeat the cycle until you are tested a preset number of cavities. Also presented methods for automatic selection of drugs in which the set to select compounds that activate, inhibit or enhance the activity USED THE RECIPIENT OR CELLULAR ion channel. TESTS ARE BASED ON TESTS ON FLUORESCENT INDICATORS USED CONTAINING VIABLE CELLS IN THEIR CYTOPLASM actual levels of a reporter fluorescent RESPONDS TO RELATED TO CHANGES TO THE CONCENTRATION OF IONS. Activation of ion channels or receptors BEGINS IN THE APPARATUS OF AUTOMATIC MEASUREMENT BY INJECTION IN ONE OR MORE OF THE CAVES DEFAULT CONTAINING CELLS A reagent is a known activator or putative ion channel OR RECIPIENTS OF INTEREST. ACTIVITY RESULTING FROM ion channels or receptors (causing CHANGES IN THE CONCENTRATION OF IONS CYTOPLASM) is determined by measuring changes in fluorescence intensity FLAG in response to a wavelength of excitement.