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METHOD FOR INFLUENCING THE POLLEN DEVELOPMENT BY MODIFYING THE SUCROSE METABOLISM

机译:通过修饰蔗糖代谢影响花粉发育的方法

摘要

The invention relates to a method for influencing the pollen development by modifying the sucrose metabolism in transgenic plant cells and plants. The invention relates especially to a method for generating male sterile plants by sucrose depletion in pollen. The invention relates particularly to the expression of a protein having the enzymatic activity of a sucrose isomerase in transgenic plant cells. The invention also relates to nucleic acid molecules that contain a DNA sequence encoding a protein having the enzymatic activity of a sucrose isomerase, wherein the DNA sequence is functionally linked with the regulatory sequences of a promoter active in plants so that the DNA sequence is expressed in anthers or pollen. The invention further relates to transgenic plants and plant cells that contain the inventive nucleic acid molecule and whose male plant and plant cells are sterile due to the expression of the DNA sequence that encodes a protein having the enzymatic activity of a sucrose isomerase. The invention also relates to the harvest products and the propagation material of said transgenic plants.
机译:本发明涉及通过修饰转基因植物细胞和植物中的蔗糖代谢来影响花粉发育的方法。本发明尤其涉及通过花粉中蔗糖消耗产生雄性不育植物的方法。本发明尤其涉及在转基因植物细胞中表达具有蔗糖异构酶的酶促活性的蛋白质。本发明还涉及含有编码具有蔗糖异构酶的酶活性的蛋白质的DNA序列的核酸分子,其中该DNA序列与植物中有活性的启动子的调节序列功能性连接,从而在植物中表达该DNA序列。花药或花粉。本发明进一步涉及含有本发明核酸分子的转基因植物和植物细胞,其雄性植物和植物细胞由于编码具有蔗糖异构酶酶活性的蛋白质的DNA序列的表达而是无菌的。本发明还涉及所述转基因植物的收获产物和繁殖材料。

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