MONOCLONAL ANTIBODIES AGAINST SOLUBLE TNF-ALPHA RECEPTORS p55 and p75 AS WELL AS AGAINST TNF-ALPHA AND ITS ANALOGUES

摘要

The present invention belongs to the field of pharmaceutical industry and relates to novel monoclonal antibodies and fragments thereof which are bound to the available surface of a human recombinant TNF-α, TNF-α analogues and TNF-α soluble receptors p55 and p75. Further, the invention relates to stable hybridoma cell lines, which lines are capable of producing such monoclonal antibodies, to a process for the preparation thereof and to pharmaceutical preparations containing them. The invention also relates to a method for the quantitative determination of human TNF-α, free or complexed with soluble TNF-α receptors p55 and p75, and to a kit used therefor. By hitherto disclosed monoclonal antibodies and reagents developed therefrom, it has not been possible to simultaneously determine free TNF-α and its soluble receptors p55 and p75 as well as complexes thereof. Another object of the invention is also the TNF-α analogue TNF-α Cys95His107His108Cys148, which in a complex with TNF-α soluble receptors, is used as an antigen for immunization of mice.