The procedure for stabilising an individual substrate for spectroscopic detection of malignancy according to this invention, having as starting components 18.4-20 ml exudate from pleural effusions or pericardial effusions or ascites, 0.3-1.2 ml of stabiliser for separating turbid and clear centrifugates, 0.3-0.7 ml of a transparent solvent, such as ethanol, dioxan or tetrahydrofuran, of spectroscopic purity, with a novelty in taking from human body in a sterile manner and as customary the quantity of 18.4-20 ml as exudate, composed of pleural effusions or pericardial effusions or ascites, to which 0.3-1.2 ml of 10 mass % aqueous solution fo guanidinium sulphate, cesium sulphate, potassium dihydrogen phosphate or ammonium sulphate is added, and the obtained mixture is centrifuged for 800-1050 s at 1370-1600 rpm, whereby an aliquot part of 2.2-2.7 ml is taken from the overall volume of the obtained clear sample, to which 0.3 - 0.7 ml of transparent solvent (Spectroscopic purity), ethanol, is added and the individual substrate sample obtained is frozen at 248-257ÕK, until immediately used for spectroscopic detectionof malignancy. A new diagnostic method for detecting malignancy is defined in this manner.
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机译：根据本发明的用于光谱学检测恶性肿瘤的稳定单个底物的方法，其具有作为起始成分的18.4-20ml来自胸腔积液或心包积液或腹水的渗出液，0.3-1.2ml用于分离浑浊和透明离心物的稳定剂，0.3-1.2ml稳定剂。 0.7毫升光谱纯度纯净的透明溶剂，例如乙醇，二恶烷或四氢呋喃，具有以无菌方式从人体中摄取的新颖性，按惯例，由胸膜积液或心包膜形成的渗出液的量为18.4毫升至20毫升向其中加入0.3-1.2 ml 10质量％的硫酸胍，硫酸铯，磷酸二氢钾或硫酸铵的积液或腹水，并将所得混合物在1370-1600 rpm下离心800-1050 s，从而从获得的澄清样品的总体积中取出2.2-2.7毫升的等分试样，在其中加入0.3-0.7毫升透明溶剂（光谱纯度），乙醇添加，将得到的单个底物样品冷冻在248-257ÕK下，直到立即用于恶性肿瘤的光谱检测。以这种方式定义了一种用于检测恶性肿瘤的新诊断方法。
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