METHOD FOR CONSTRUCTION OF BACTERIAL STRAINS PRODUCING AMINO ACIDS (VARIANTS) AND METHOD FOR PREPARING AMINO ACID BY FERMENTATION USING CONSTRUCTED BACTERIAL STRAINS PRODUCING AMINO ACIDS

摘要

FIELD: biotechnology, microbiology, amino acids. SUBSTANCE: invention relates to method for construction of strains of coryneform microorganism eliciting the enhanced productivity of amino acids and to method for preparing amino acid by fermentation using constructed strains of coryneform microorganisms. Method for construction of strains of coryneform microorganisms involves insertion of mutation in promoter sequence of genes that encode biosynthesis of amino acid on chromosome of coryneform microorganism to obtain mutant of coryneform microorganism followed by culturing prepared mutant and selection of mutant able to produce require amino acid in large amounts. Invention provides preparing the high yield of amino acids by enhancement and regulation of expression of required gene being without plasmid using. EFFECT: improved preparing method. 23 cl, 26 tbl, 3 dwg, 7 ex