Characterizing large numbers of samples with volume in microliter or sub-microliter range in parallel uses solutions containing two different indicators to produce signals of different intensities

摘要

Characterizing large numbers of samples with a volume in the microliter or sub-microliter range in parallel comprising using micropipettes to dispense samples or reagents into a microtiter plate (each sample or reagent containing two indicators with different optical properties), determining the optical signals produced by reach diluted sample or reagent and obtaining an average value for signal intensity for all the samples, is new. Characterizing large numbers of samples with a volume in the microliter or sub-microliter range in parallel uses micropipettes to dispense samples or reagents into a microtiter plate. The volume of each sample or reagent is determined in the pipette system under conditions of highly reduce evaporation. Each sample or reagent contains two indicators with different optical properties and is mixed with a diluent containing one indicator in an identical concentration. The optical signals produced by reach diluted sample or reagent in a microwell are determined and are corrected to compensate for the geometry of the well, surface of the sample, etc. An average value is obtained for signal intensity for all the samples and the deviation of each sample from this is calculated. An Independent claim is included for a test kit for carrying out the method comprising two solutions, each containing two indicators, for use in fluorescence analysis, two similar solutions for photometric analysis and a temperature-independent buffer solution.