Stem-like cells or embryos can be produced by interspecies nuclear transfer

摘要

With (in the same species as the donor) or mitochondrial compatible cytoplasmic and / to provide a nuclear transfer method for implantation into oocytes which were enucleated animal species different from the donor cell, the nucleus of the donor cells differentiated . Nuclear transfer unit obtained is useful embryonic stem cells isogenic, in helping to create the stem cells or embryonic cells of isogenic humans. In creating a differentiated cell of interest, and for example introducing a gene of interest in a specific site of the genome of these cells by homologous recombination, stem-like cells or embryos these cells, on modifying or removing , are useful. May contain a heterologous gene, these cells are particularly useful for the study of cell differentiation in in vitro and cell transplantation therapy. By introducing (similar species or same species as donor cell or nucleus) mitochondrial DNA, may increase the efficiency of nuclear transfer can be compatible or cytoplasm. Furthermore, by culturing under conditions that promote the growth and development of the embryo and / or by selecting a cell cycle specific, that it is genetically engineered donor cells to inhibit apoptosis, nuclear transfer efficiency It is possible to further increase.