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High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications

机译:高水平表达和蛋白,肽和缀合物的简便纯化,可用于免疫,纯化和检测应用

摘要

A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.
机译:描述了产生和纯化肽以及产生用于抗体产生的肽/蛋白质抗原的方法。该方法利用融合蛋白,特别是融合蛋白,其中融合蛋白载体区段包括至少约65个氨基酸长的氨基酸序列,并且其中氨基酸序列不包含氨基酸的带负电或带正电的侧链。

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