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Culturing encapsulated chondrocytes under reduced oxygen partial pressure to produce cartilage implants
Culturing encapsulated chondrocytes under reduced oxygen partial pressure to produce cartilage implants
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机译:在降低的氧气分压下培养封装的软骨细胞以生产软骨植入物
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摘要
A process is provided for the production of a human cartilage implant from chondrocytes cultured in vitro, which come as close as possible to the original with respect to their biochemical composition and biomechanical properties. Up to 20% vol. of human serum is used as medium addition in the process. The chondrocytes can be kept in monolayer culture until the 12th passage in order firstly to be re-differentiated, incubated under a reduced oxygen partial pressure, and subsequently stimulated to form a three-dimensional cartilage tissue due to aggregation under an oxygen partial pressure of 21%. In an embodiment, chondrocytes in alginate beads are cultured in a nutrient solution, which may contain human serum and one or more chondrogenic growth factors, under an oxygen partial pressure of less than 20 volume %, isolated from the alginate beads by a treatment with a chelating agent, aggregated by centrifugation and cultured under an oxygen partial pressure of: −21 volume %.
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机译:提供了一种由体外培养的软骨细胞生产人软骨植入物的方法,就其生化组成和生物力学性质而言,其与原始软骨细胞尽可能接近。最高20%体积在此过程中,将人血清的一半用作培养基添加。可以将软骨细胞单层培养直至第十二次传代,以便首先重新分化,在降低的氧分压下温育,然后刺激以形成三维软骨组织。在21%的氧气分压下聚集。在一个实施方案中,在小于20体积%的氧分压下,通过用H 2 O 3处理从藻酸盐珠分离的藻酸盐珠粒中的软骨细胞在营养溶液中培养,所述营养液可包含人血清和一种或多种软骨生成生长因子。螯合剂,通过离心聚集并在氧气分压为-21体积%的条件下培养。
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