The bacterial cell, method for the production of a heterologous polipeptu00ecdeo, method for preventing cleavage methods for the cleavage and methods for the production of a polipeptu00ecdeo cleaved

摘要

"The bacterial cell, method for the production of a heterologous polipeptu00ecdeo, method for preventing cleavage methods for the cleavage and methods for the production of a polipeptu00ecdeo cleaved.It is described a bacterial cell gram negative which is deficient of a chromosomal gene is present in a cell of a wild type.In which the gene shares at least 80% identity with the sequ00b3u00eancia sequ00b3u00eancia native gene encodes a yfck and aminopeptidaso.Alternatively, a gram negative bacterial cell is deficient in a gene present in this cell chromosomal wild-typeIn which the gene encodes a aminopeptidase that shares at least 80% identity with the sequ00b3u00eancia sequ00b3u00eancia native aminopeptidase b2324.Any of these types of cells, when understanding a codec of a heterologous polypeptide nucleic acid.Produces a polypeptide not assigned n - terminal when it is grown, and the polypeptide is recovered, with virtually no prominent polypeptide N-terminus produced as an impurity.On the other hand,It is provided a method for the cleavage of amino acid N - terminal of a polypeptide which comprises the contact of the polypeptide with aminopeptidase that shares at least 80% identity of sequ00b3u00eanciaWith the sequ00b3u00eancia native aminopeptidase b2324.