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USE OF NUCLEIC ACID ANALOGUES IN THE INHIBITION OF NUCLEIC ACID AMPLIFICATION
USE OF NUCLEIC ACID ANALOGUES IN THE INHIBITION OF NUCLEIC ACID AMPLIFICATION
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机译:核酸类似物在抑制核酸扩增中的应用
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PCT No. PCT/EP93/01435 Sec. 371 Date Mar. 10, 1995 Sec. 102(e) Date Mar. 10, 1995 PCT Filed Jun. 7, 1993 PCT Pub. No. WO93/25706 PCT Pub. Date Dec. 23, 1993Nucleic acid analogues such as peptide-nucleic acids (PNAs) which hybridize strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridizing a PNA to PCR amplification products. Assays capable of discriminating between single base mutants are conducted by using a PNA hybridizing to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asymmetric PCR amplifications are carried out by starting a PCR symmetrically using like quantities of forward and reverse primers, and, once the amplification is established, disabling one primer by hybridizing a PNA thereto.
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