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CELLULAR TEST SYSTEM FOR EXAMINING THE METABOLIC ACTIVITY OF ACTIVE FORMS OF THE CYTOCHROM P450 2D6
CELLULAR TEST SYSTEM FOR EXAMINING THE METABOLIC ACTIVITY OF ACTIVE FORMS OF THE CYTOCHROM P450 2D6
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机译:用于检测细胞色素P450 2D6活性形态代谢活性的细胞测试系统
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摘要
Test system comprising cells expressing a cytochrome P450 2D6 (hCYP2D6) allele heterolog, and at least three P450 2D6 alleles are expressed in the system, is new. Independent claims are also included for the following: (1) screening for substances metabolized by human cytochrome P450 2D6, comprising contacting cells of the test system with a substance and measuring metabolite products; (2) determining new P450 2D6 alleles, comprising heterologous expression of the allele in question in a cell and comparing P450 2D6-dependent metabolism in this cell with cells of the test system; and (3) quantifying cytochrome P450 content, comprising solubilizing P450 cytochromes with a non-ionizing detergent Emulgen 913, centrifugation and measurement by CO difference spectra.
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