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Procedure is for production of polypeptide in yeast and involves first stage of fermentation of a yeast cell containing a sequence of deoxyribonucleic acid codifying polypeptide of interest
Procedure is for production of polypeptide in yeast and involves first stage of fermentation of a yeast cell containing a sequence of deoxyribonucleic acid codifying polypeptide of interest
The procedure is for the production of a polypeptide in yeast and involves a first stage of fermentation of a yeast cell containing a sequence of deoxyribonucleic acid codifying a polypeptide of interest, which is transcribed under control of a promoter of pH under conditions of pH permitting cellular growth. In a second stage, the pH value is increased to augment the transcription of the polypeptide. In a third stage, fermentation occurs during a determined period of time and under the same conditions of pH as the previous stage. In a fourth stage, the polypeptide produced is isolated.
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