RECEPTOR-MEDIATED GENE TRANSFER SYSTEM FOR TARGETING TUMOR GENE THERAPY

摘要

The invention relates to a gene transfer system binding to a growth factorreceptor,comprising 4-element complex gene transfer system consisting of ligandoligopeptide (LOP)/polycationic polypeptide/endosome release oligopeptide/exogenous DNA or 3-element complexconsisting of ligand oligopeptide (LOP)/polycationic polypeptide/exogenousDNA. LOPselected from E5, GE7, GV1 and GV2 and constructed as a composite polypeptidevector canbe used to target exogenous genes into malignant tumor cells or tumor vascularendothelial cells.They are also able to highly inhibit the growth of tumor cells in animals whenp15, p16 orp21 WAF-1 is used as the exogenous genes. The invention also allows targetingtwo or more typesof exogenous genes and transducing exogenous genes to hemopietic, lymphoid,liver, kidney,nerve cells using a specific LOP that can recognize relevant receptorsexpressed in these cells forgene therapy.