首页> 外文期刊>中国癌症研究(英文版) >ENHANCED ANTITUMOR EFFECTS OF SUICIDE GENE THERAPY BY SIMULTANEOUS TRANSFER OF GMCSF GENE IN LEUKEMIA-BEARING MICE
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ENHANCED ANTITUMOR EFFECTS OF SUICIDE GENE THERAPY BY SIMULTANEOUS TRANSFER OF GMCSF GENE IN LEUKEMIA-BEARING MICE

机译:GMCSF基因同时转移对白血病小鼠的自杀基因治疗的抗肿瘤作用

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摘要

In the present report, antitumor effect of combined transfer of suicide gene and cytokine gene was studied.Adenovirus engineered to express E. Coli. Cytosine deaminase (AdCD) and/or adenovirus engineered toexpress murine granulocyte-macrophage colonystimulating factor (AdGMCSF) were used for the treatment of leukemia-bearing mice. The mice were inoculated s.c. With FBL-3 erythroleukemia cells and 3days later received intratumoral injection of AdCD in the presence or absence of AdGMCSF followed by intraperitoneal 5-fluorocytosine (5FC) treatment. The results demonstrated that mice received combined therapy of AdCD/5FC and AdGMCSF developed tumors most slowly and survived much longer when compared with mice treated with AdCD/5FC alone, AdGMCSF alone, AdlacZ/5FC or PBS. Combined transfer of CD gene and GM-CSF gene achieved higher specific CTL activity than control therapies. Pathological examination illustrated that the tumor mass showed obvious necrosis and inflammatory cell infiltration in mice after combined therapy. The results demonstrated that combined transfer of suicide gene and cytokine gene could synergistically inhibit the growth of leukemia in mice and induce antitumor immunity of the host. The combination therapy might be a potential approach for cancer gene therapy.
机译:本文研究了自杀基因和细胞因子基因联合转移的抗肿瘤作用。胞嘧啶脱氨酶(AdCD)和/或工程改造以表达鼠粒细胞-巨噬细胞集落刺激因子(AdGMCSF)的腺病毒用于治疗白血病小鼠。给小鼠皮下接种。在存在或不存在AdGMCSF的情况下,使用FBL-3红细胞白血病细胞并在3天后进行肿瘤内注射AdCD,然后进行腹膜内5-氟胞嘧啶(5FC)治疗。结果表明,与单独接受AdCD / 5FC,单独AdGMCSF,AdlacZ / 5FC或PBS治疗的小鼠相比,接受AdCD / 5FC和AdGMCSF联合治疗的小鼠发展最快的肿瘤和存活时间更长。 CD基因和GM-CSF基因的联合转移获得了比对照疗法更高的比CTL活性。病理检查表明,联合治疗后,小鼠肿块明显坏死,炎症细胞浸润。结果表明,自杀基因和细胞因子基因的联合转移可以协同抑制小鼠白血病的生长,并诱导宿主的抗肿瘤免疫力。联合治疗可能是癌症基因治疗的潜在方法。

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