METHOD FOR THE DETECTION OF PATHOGENIC GRAM POSITIVE BACTERIA FROM THE GENERA STAPHYLOCOCCUS, ENTEROCOCCUS AND STREPTOCOCCUS

摘要

The present invention is directed to a method for identification of a Grampositive pathogenic bacterium comprising (a) an amplification step with atleast a first set of amplification primers capable of amplifying a preselectednucleic acid sequence region from a first predetermined sub-group ofpathogenic Gram positive bacteria, (ab) a detection step with at least a firsthybridization reagent capable of specifically detecting a preselected nucleicacid sequence region from said first predetermined sub-group of pathogenicGram positive bacteria, said detection step comprising steps (aba) monitoring,whether hybridization has occurred at a preselected temperature, saidoccurrence of hybridization being indicative for at least the genus of thepathogenic organism present in the sample, and (abb) monitoring temperaturedependence of hybridization, said temperature dependence being indicative forat least the species of said pathogenic Gram positive bacterium.