METHOD FOR THE DETECTION OF PATHOGENIC GRAM POSITIVE BACTERIA FROM THE GENERA STAPHYLOCOCCUS, ENTEROCOCCUS AND STREPTOCOCCUS

摘要

The present invention is directed to a method for identification of a Gram positive pathogenic bacterium (eg. Staphylococcus, Streptococcus, Enterococcus) comprising (a) an amplification step with at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region (such as the 16S-23S rRNA spacer region) from a first predetermined sub-group of pathogenic Gram positive bacteria, (b) a detection step with at least a first hybridization reagent capable of specifically detecting a preselected nucleic acid sequence region from said first predetermined sub-group of pathogenic Gram positive bacteria, said detection step comprising steps (ba) monitoring, whether hybridization has occurred at a preselected temperature, said occurrence of hybridization being indicative for at least the genus of a pathogenic organism present in the sample, and (bb) monitoring temperature dependence of hybridization (using a pair of FRET-probes complementary to adjacent sequences), said temperature dependence being indicative for at least the species of said pathogenic Gram positive bacterium.