ANTI-IDIOTYPE ANTIBODY, METHOD OF CONSTRUCTING THE ANTI-IDIOTYPE ANTIBODY AND METHOD OF PREPARING IDIOTYPE ANTIBODY USING THE ANTI-IDIOTYPE ANTIBODY

摘要

It is intended to provide an anti-idiotype antibody which can be conveniently and economically constructed compared with the existing type; a method of constructing the anti-idiotype antibody; and a method of preparing a target idiotype antibody using the above-described anti-idiotype antibody. A substance binding to the antigen-binding site of a first antibody is prepared and this substance is ligated to a second antigen to give a fused antigen. Next, this fused antigen is bonded to a second antibody capable of binding to the second antigen as described above, thereby giving an anti-idiotype antibody against the first antibody. In a method of preparing a specific idiotype antibody which comprises inoculating an animal with an anti-idiotype antibody to the idiotype antibody and then further inoculating with an antigen of the above idiotype antibody, the anti-idiotype antibody as described above is employed. Thus, the target idiotype antibody can be efficiently obtained.