Anti-idiotype antibody, method of constructing the anti-idiotype antibody, and method of preparing idiotype antibody using the anti-idiotype antibody

摘要

The present invention provides: an anti-idiotype antibody which can be conveniently and economically produced compared with the existing type; a method of producing the anti-idiotype antibody; and a method of preparing a target idiotype antibody using the anti-idiotype antibody. A substance capable of binding to an antigen-binding site of a first antibody is prepared and this substance is ligated to a second antigen to give a fused antigen. Next, this fused antigen is bonded to a second antibody capable of binding to the second antigen, thereby giving an anti-idiotype antibody against the first antibody. In a method of preparing the idiotype antibody including: inoculating an animal with an anti-idiotype antibody against a specific idiotype antibody; and then further inoculating the animal with an antigen of the above idiotype antibody, the anti-idiotype antibody of the present invention is employed. Thus, the target idiotype antibody can be efficiently obtained.