Optimizing giant unilamellar vesicle (guv) growth in a physiological buffer and artificial cell based biosensor

摘要

The yield of giant unilamellar vesicles (GUVs) is greatly increased by influencing the characteristics of the lipid film. Lipid films are formed in containers made from hydrophobic material that have a low affinity for hydrated lipid films, preferably TEFLON test tubes. Additionally, the lipid film is dried rapidly using a vortex-drying process. The GUVs that are formed may be incorporated into an artificial cell based biosensor. The biosensor includes an addressable array containing a plurality of host cavities for holding the GUVs, preferably functionalized GUVs.