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METHOD OF SEPARATING EPITHELIAL CELLS FROM A HUMAN OR ANIMAL URINE SAMPLE

机译:从人或动物尿液样本中分离上皮细胞的方法

摘要

The invention relates to a method of separating epithelial cells from a human or animal urine sample containing said cells. The inventive method is characterised in that it comprises the following steps: (a) microfiltration of the urine sample on a membrane filter with an average pore diameter of 11 µm, in order to obtain a microfiltration retentate containing mostly epithelial cells, and (b) recovery of the cells from the microfiltration retentate by treating the membrane filter with a solution containing trypsin.
机译:本发明涉及从含有所述细胞的人或动物尿液样品中分离上皮细胞的方法。本发明方法的特征在于其包括以下步骤:(a)在平均孔径为11μm的膜滤器上对尿液样品进行微滤,以获得主要包含上皮细胞的微滤滞留物,和(b)通过用含有胰蛋白酶的溶液处理膜过滤器,从微滤渗余物中回收细胞。

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