CELLOBIOHYDROLASE REDUCED GLYCOSYLATION VARIANTS: CBHIN45A; CBHIN270A; AND CBHIN384A

摘要

The invention provides a method for making an active exoglucanase in a eukaryotic heterologous host, the method comprising reducing glycosylation of the exoglucanase, wherein reducing comprises replacing an N-glycosylation site amino acid residue with non-glycosyl accepting amino acid residue. The invention further provides a cellobiohydrolase, comprising the reduced glycosylation variant cellobiose enzymes CBHIN45A; CBHIN270A; or CBHIN384A, or any combination thereof.