10241 Method for preparation of exo-polymers from submerged mycelial culture of Ganoderma lucidum TGKCTC 10241BP and the use thereof

摘要

PURPOSE: A method for preparing exo-polymers from the mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) and the use thereof are provided. The exo-polymers increase glycogen concentration in muscle and the liver of a mouse, decrease lactic acid accumulation in the serum of a mouse and increase movement of a mouse. CONSTITUTION: A method for preparing exo-polymers from the mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) comprises the steps of: (a) inoculating a mycelium of Ganoderma lucidum TG(KCTC 10241BP) in a synthetic medium and culturing at 25 deg. C and 100 to 150 rpm for 5 to 15 days; (b) homogenizing the cultured medium in ice water; (c) inoculating the homogenized medium to another synthetic medium and culturing it under conditions of 1vvm, pH 4 to 7, 20 to 35 deg. C and 50 to 150 rpm for 10 to 30 days; (d) centrifuging the medium at 5,000 to 15,000xg for 10 to 30 minutes and isolating the supernatant; and (e) treating the supernatant with ethanol, dissolving the precipitate in distilled water, centrifuging the solution at 5,000 to 15,000xg for 10 to 30 minutes to isolate the supernatant and dialyzing the supernatant.