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A process for preparation of exo-polymers from submerged mycelial culture of Ganoderma lucidum and the use of the same

机译:从灵芝的深层菌丝培养物中制备外聚合物的方法及其用途

摘要

The invention Reishi mushroom (Ganoderma lucidum) in the production method of the polymer material (Exo-polymer) from the culture mycelia, and their use to inoculate Ganoderma mycelium on synthetic medium Ganoderma lucidum and pH 4~7, 25~35 ℃ temperature on while supplying air at a rate of 1vvm by stirring at 10~30 50~150rpm ilgan liquid culture, and then obtaining a precipitate was separated by the centrifugal 10-30 minutes to the culture medium to 5,000~15,000 × g supernatant was treated in ethanol and freeze-dried a polymer material isolated by dissolving the following: distilled water, separated and the dialysis centrifuge consists of protein 17.2% and carbohydrate 82.8%, and it contains a glycoprotein with a molecular weight of 780kDa, increasing the glycogen concentration in muscle and liver upon administration to the mouse, reduce the accumulation of lactic acid in serum and, an excellent effect of enhancing the locomotion.
机译:灵芝蘑菇(灵芝)在培养菌丝体中制备高分子材料(Exo-polymer)的方法,以及在合成培养基灵芝和pH 4〜7上接种灵芝菌丝体的用途。 ,在25〜35℃的温度下边搅拌边以10v至30rpm的速度向ilgan液体培养物中以1vvm的速度供应空气,然后通过离心分离10-30分钟获得的沉淀物向培养基中分离至5,000〜15,000 ×g上清液在乙醇中处理,然后冷冻干燥通过溶解以下物质分离出的聚合物材料:蒸馏水,分离,透析离心机由17.2%的蛋白质和82.8%的碳水化合物组成,并且包含分子量为780kDa的糖蛋白,在给小鼠服用后,可增加肌肉和肝脏中糖原的浓度,减少血清中乳酸的积累,并具有增强运动的出色效果。

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