Specific Primers for Detection of Pepper mild mottle virus

摘要

PURPOSE: Specific primers for detecting pepper mild mottle virus are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of pepper mild mottle virus, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting pepper mild mottle virus comprise a nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 4 which species specifically binds with pepper mild mottle virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequence set forth in SEQ ID NO: 5 or SEQ ID NO: 6 which is combined with the selected nucleic acid and species specifically binds with pepper mild mottle virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.