MYCOBACTERIUM TUBERCULOSIS SPECIFIC PROTEIN HAVING EXCELLENT SENSITIVITY AND SPECIFICITY TO TUBERCULOSIS PATIENTS

摘要

PURPOSE: Provided is a method for producing new protein Rv3369 containing 145 amino acids by using a microorganism transformed with an expression vector containing the gene encoding the new protein Rv3369. The Rv3369 has excellent sensitivity and specificity to tuberculosis patients and thus can be widely used for the diagnosis of tuberculosis. CONSTITUTION: New protein Rv3369 (sequence number 1) detected in Myco-bacterium tuberculosis contains 145 amino acids. A gene Rv3369 (sequence number 2) contains 435bp sequences and encodes the new protein. An Escherichia coli expression vector pRSETrv3369 contains the gene Rv3369. The Rv3369 is obtained by culturing transformed E. coli (pRSET/BL21)(Escherichia coli 3369 (pRSET/BL21))(KCCM10462). A diagnostic kit for the detection of M. tuberculosis infection contains an Rv3369-absorbed plate, a detector for detection of a reaction, a substrate liquid for detector, positive control serum, negative control serum, dilute and washing solutions for specimens.