PRIMERS FOR DETECTING FRUIT TREE INFECTING VIRUS INFECT AND SCREENING METHOD OF VIRUS USING SAME

摘要

PURPOSE: Primers for detecting fruit tree infecting virus and a screening method of fruit tree infecting virus using the same are provided, thereby easily detecting infection of ASGV(apple stem grooving capillovirus), ACLSV(apple chlorotic leafspot trichovirus), CTV(citrus tristeza virus) and CTLV(citrus tatter leaf virus). CONSTITUTION: Primers for detecting fruit tree infecting virus are selected from the group consisting of ASGV-Pf primer having the nucleotide sequence of SEQ ID NO: 1, ASGV-Pr primer having the nucleotide sequence of SEQ ID NO: 2, ACLSV-CPf primer having the nucleotide sequence of SEQ ID NO: 3, ACLSV-CPr primer having the nucleotide sequence of SEQ ID NO: 4, CTLV-CPf primer having the nucleotide sequence of SEQ ID NO: 5, CTLV-CPr primer having the nucleotide sequence of SEQ ID NO: 6, CTV-CPf primer having the nucleotide sequence of SEQ ID NO: 7, and CTV-CPr primer having the nucleotide sequence of SEQ ID NO: 8. A method for screening fruit tree infecting virus comprises the steps of: (a) amplifying RNA extracted from a sample as a template using RT-PCR using one primer selected from ASGV-Pf primer, ASGV-Pr primer, ACLSV-CPf primer, ACLSV-CPr primer, CTLV-CPf primer, CTLV-CPr primer, CTV-CPf primer, and CTV-CPr primer; and (b) analyzing the size of PCR products.