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PRIMERS FOR DETECTING ORCHID INFECTING VIRUSINFECT AND SCREENING METHOD OF VIRUS USING SAME
PRIMERS FOR DETECTING ORCHID INFECTING VIRUSINFECT AND SCREENING METHOD OF VIRUS USING SAME
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机译:兰花感染病毒检测的主要方法和病毒筛查方法
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摘要
PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
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