The bacterial strain Vibrio cholerae KM 207 classic biovar Serovar Inaba - producer of protective antigens

摘要

FIELD: biotechnology, microbiology. SUBSTANCE: invention relates to preparing the strain Vibrio cholerae KM 207 of classic biovar serovar Inaba as a producer of protective antigens (subunit B as component of choleraic toxin, the main colonizing factor of choleraic vibrio, toxin-coregulated adhesion pili, protective and immunogenic protein OmpU of external membrane). Method involves conjugative incorporation to cells the natural toxigenic strain Vibrio cholerae 569B of classic biovar serovar Inaba recombinant plasmid pCT105 (KmrTcr) with cloned genes of choleraic toxin and the following elimination of plasmid. The level of choleraic toxin production in the strain is increased by 2.6 times (23.4 mcg/ml), synthesis of toxin- -coregulated adhesion pili is elevated also and protein OmpU appeared. In culturing the strain addition of antibiotics to medium is not required as in the case of plasmid strains. Using the strain allows the enhancement of choleraic toxin production level, toxin-coregulated adhesion pili (this causes self-agglutination of cells in broth) and synthesizes protein OmpU of external membrane. Invention can be used for preparing vaccines and diagnostic test-systems. EFFECT: valuable properties of strain. 2 ex