Screening and Identification of a Bacterial Strain Antagonistic to Vibrio parahaemolyticus

摘要

In the present study,bacterial strains were isolated from the shrimp(Penaeus orientalis)ponds in different regions of Hainan Province,and tested for their antagonistic activity against Vibrio species on 2216E plates,using V.parahaemolyticus,V.harveyi and V.anguillarum as the indicator bacteria.As a result,an antagonistic strain was screened out and numbered 20160522Z-10(referred to as Z-10).Then,the antibacterial activity and minimum inhibitory concentration(MIC)of Z-10 against the indicator bacteria,and its antibacterial effect against other six Vibrio species were determined.The results proved that Z-10 was antagonistic to all the three indicator bacteria,and its MIC against V.parahaemolyticus,V.harveyi and V.anguillarum was 2.5×10~4,2.5×10~5 and 2.5×10~5 CFU/ml.Additionally,Z-10 had a certain antibacterial effect against other Vibrio species.Finally,the strain was identified as a Pseudoalteromonas sp.by 16S rDNA sequence analysis.The bacterium as a new probiotic,is expected to be used in disease prevention and control in marine aquaculture.