RIBONUCLEIC ACID-AMPLIFYING REAGENT COMPOSITION CAPABLE OF DIMINISHING FALSE POSITIVE, AND METHOD FOR AMPLIFYING RIBONUCLEIC ACID USING THE COMPOSITION

摘要

PPROBLEM TO BE SOLVED: To provide a ribonucleic acid-amplifying reagent composition capable of easily diminishing false positive, to provide a method for amplifying a ribonucleic acid using the composition, and to provide a reagent or kit for the method. PSOLUTION: The ribonucleic acid-amplifying reagent composition capable of amplifying in a single step a ribonucleic acid using at least one enzyme comprises (a) a component for splitting a deoxyribonucleic acid contained in a sample by an enzyme having DNA-splitting activity, (b) a component for converting to a deoxyribonucleic acid a ribonucleic acid contained in the sample by an enzyme having reverse transcription activity, and (c) a component for amplifying a deoxyribonucleic acid complementary to a target RNA by an enzyme having DNA polymerase activity. The method for amplifying a ribonucleic acid using the composition and the reagent or kit for the method are also provided, respectively. PCOPYRIGHT: (C)2006,JPO&NCIPI