METHODS FOR SCREENING ENZYME FOR MODIFYING VALOSIN-CONTAINING PROTEIN AND SUBSTANCE FOR ADJUSTING THEIR ENZYMATIC ACTIVITIES

摘要

PROBLEM TO BE SOLVED: To provide a screening method for specifying a phosphorylation enzyme or a dephosphorylation enzyme for the 765-Ser residue of a valosin-containing protein (VCP) and an acetylation enzyme or a deacetylation enzyme for the 696-Lys residue, and to provide a screening method for specifying an activating substance and an inhibitory substance for the enzymes.;SOLUTION: The former screening method comprises bringing a VCP sample as a reaction substrate into contact with a candidate enzyme and/or a candidate substance and then measuring a phosphorylation or an acetylation at the prescribed amino acid residues (S765 and K696) of the VCP sample.;COPYRIGHT: (C)2005,JPO&NCIPI