Glutamine auxotrophic human cells with the ability to grow in glutamine-free medium and has the ability to produce the protein

摘要

Were transfected with exogenous DNA sequences encoding the glutamine synthetase and exogenous DNA sequences capable of altering the expression of an endogenous gene encoding the protein or exogenous DNA sequence encoding a protein A glutamine-auxotrophic human cells, the exogenous DNA sequences they will be located in the DNA construct on one or more than one cells the transfected glutamine-free medium and has the ability to produce the protein cells that have the ability to grow in the middle.