GLUTAMINE-AUXOTROPHIC HUMAN CELLS CAPABLE OF PRODUCING PROTEINS AND CAPABLE OF GROWING IN A GLUTAMINE-FREE MEDIUM

摘要

PPROBLEM TO BE SOLVED: To provide a glutamine-auxotrophic human cell capable of producing proteins and capable of growing in a glutamine-free medium. PSOLUTION: Disclosed is a glutamine-auxotrophic human cell transfected with an exogenous DNA sequence capable of altering the expression of an exogenous DNA sequence encoding a protein or an endogenous gene encoding a protein and with an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more than one DNA construct, and the transfected cell is capable of producing the protein and capable of growing in a glutamine-free medium. PCOPYRIGHT: (C)2008,JPO&INPIT