FLUORESCENCE MICROSCOPE AND EXCITING LIGHT SOURCE CONTROL METHOD THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a fluorescent microscope which allows the intensity of exciting light to be controlled by detecting an accurate intensity of exciting light in an observation position.;SOLUTION: The fluorescent microscope is provided with; a light source 1 for generating exciting light, a first light intensity detector 3 which is arranged integrally with the light source 1 and measures the intensity of exciting light; an illuminating optical system 6 for radiating exciting light to a sample arranged in the observation position; an observation system for observing fluorescence emitted from the sample, resulting from radiation of exciting light; and a second light intensity detector which measures the intensity of exciting light in the observation position while the sample is caused to recede from the observation position, and a detection value of the first light intensity detector and that of the second light intensity detector are associated with each other, so that the intensity of exciting light of the light source 1 can be controlled by using the first light intensity detector.;COPYRIGHT: (C)2005,JPO&NCIPI