Being manner in order to decide the nuclear acid array tonnage in the sample and manner in order to decide the nuclear acid

摘要

The quantity of a nucleic acid sequence in a sample is determined by measuring, during a time interval, related indicia in amplified calibration and test samples. Points during this interval at which the indicia in the calibration samples equal first and second cut-off levels are determined. Using a statistical criterion it is decided which of the two sets of points represents the known quantities of the sequence in the calibration samples. From this selection the quantity in the test sample is determined. Also claimed are: (1) a computer readable program for performing the determinations above; and (2) apparatus for determining a quantity of a nucleic acid sequence in a test sample by measuring the indicia and a device storing a computer program as above.