Being the manner which decides the base array of the nuclear acid of decisive mannered null multiple genera of nuclear acid base array

摘要

PPROBLEM TO BE SOLVED: To provide an analysis method by which a base sequence can be analyzed in high work efficiency, when using a nucleic acid bound to a substrate as a primer, performing an elongation reaction using the nucleic acid of analysis target as a template, and then utilizing the MALDI-TOF MS analysis of the elongation product. PSOLUTION: When a nucleic acid used as a primer is bound to a substrate, a partial structure cleavable with light is disposed in the 5'-side portion of the primer not affecting a hybridization with the template. After an elongation reaction, prescribed light is irradiated to selectively cleave the partial structure cleavable with the light. PCOPYRIGHT: (C)2004,JPO