Nucleotide sequence analysis of oligonucleotides and nucleic acids

摘要

PURPOSE: To provide a new oligonucleotide having a specific region complementary to a target sequence in a specimen nucleic acid and a non-specific region connected to at least one of both terminals of the specific region and useful e.g. for the determination of the base sequence of a nucleic acid and the diagnosis of infectious diseases and genetic diseases. ;CONSTITUTION: This oligonucleotide contains a specific region and one or two non-specific regions connected to at least one of both terminals of the specific region. The specific region has a specific base sequence complementary to the target sequence in the specimen nucleic acid active to hybridize the oligonucleotide and the non-specific region is composed of at least one nucleotide or its oligomer having the other base capable of forming base pairs for each of the bases constituting a conventional nucleic acid. This new oligonucleotide is suitable as a hybridization probe for the determination of the base sequence of a nucleic acid, the diagnosis of infectious diseases and genetic diseases and the base sequence analysis, etc., of nucleic acid such as genom mapping.;COPYRIGHT: (C)1996,JPO