Method of manufacturing a probe and method for detecting mismatches method for detecting a target nucleic acid probe, the double-stranded nucleic acid hybrids in

摘要

PURPOSE: To obtain a method for detecting a nucleic acid hybrid utilizing hybridization consisting of a more simplified process without requiring separation of B/F and capable of obtaining good measuring sensitivity. ;CONSTITUTION: Analysis of a sample is carried out using a probe to which two kinds of pigments causing an optical change capable of detecting by charge transfer through a double helix structure in a target single-stranded nucleic acid.probe.hybrid are bound in a prescribed interval to detect whether a target nucleic acid exists in the sample or not.;COPYRIGHT: (C)1994,JPO&Japio