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Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
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机译:缺乏错配修复的宿主细胞及其在使用单链核酸诱导同源重组的方法中的用途
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摘要
Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
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