Early detection of cancer of specific type using 1HNMR metabonomics

摘要

A method for determining whether a patient has a particular type of cancer which comprises: a. obtaining an ascites, abnormal cell cellular fluid or serum sample from the patient; b. diluting the sample with D₂O; c. subjecting the sample to ¹HNMR to obtain a series of free induction decay outputs (FID's); d. mathematically modifying the data to obtain ¹HNMR spectra; e. correcting the ¹HNMR spectra for phase and baseline distortions; f. data reducing the corrected ¹HNMR spectra to obtain a plurality of integral spectral segments; g. compensating for effects of variation in suppression of water resonance; h. normalizing the resulting data to total spectral area to obtain normalized ¹HNMR spectra; i. subjecting the normalized ¹HNMR spectra to principal component analysis to obtain normalized data; and j. plotting and comparing the normalized data with corresponding control data indicating the presence of the particular type of cancer to determine whether the sample indicates that the patient has the particular type of cancer.