Transfecting osteogenic precursor cells DNA encoding a LIM mineralization protein for a method of inducing bone formation

摘要

Described are isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. Further described are vectors comprising splice variants of nucleotide sequences that encode LMP, as well as isolated host cells comprising those vectors. Moreover, medicaments for methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding splice variants of LIM mineralization protein are provided. The transfection may occur ex vivo or in vitro by direct injection of virus or naked plasmid DNA. Described in particular is one method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, provided are methods for inducing systemic bone formation by stable transfection of host cells with the vectors described.