NOVEL CARBONYL REDUCTASE, GENE THEREOF AND USE OF THE SAME

摘要

The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3'-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same.   The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4):(1) activity: acting on 2-chloro-1-(3'-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to form (R)-2-chloro-1-(3'-chlorophenyl)ethanol;(2) optimum pH for activity: 5.0 to 6.0;(3) optimum temperature for activity: 40°C to 50°C;(4) molecular weight: about 40,000 as determined by gel filtration analysis, about 30,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.