EFFICIENT METHOD OF FUNCTIONAL ANALYSIS SCREENING FOR PROTEIN UTILIZING FLUORESCENCE GENERATED IN CELL-FREE PROTEIN SYNTHESIZING SYSTEM

摘要

A method of detecting any reaction between a fluorescently-labeled protein synthesized by a cell-free protein synthesizing system and a sample solution simply with high accuracy in a short period of time. Referring to a case of detecting any binding reaction between an antibody with GFP and a sugar on nanoparticle surface, in a well of microplate (3), a solution containing antibody with GFP (1) is mixed with a solution containing nanoparticle (2) having a sugar reactive with the antibody with GFP attached to the surface thereof, thereby producing mixed solution (A). After reaction thereof, FCS measuring is carried out.