Screening of Host Proteins Interacting with PorcineEpidemic Diarrhea Virus （PEDV） N Protein by YeastTwo-hybrid System

摘要

[Objective] The paper was to obtain host proteins interacting with porcine epidemic diarrhea virus(PEDV) N protein. [Method] The recombinant vector pGBKT7-N of PEDV N gene was constructed and used as the bait plasmid to screen the proteins interacting with N protein of PEDV from the cDNA library of porcine alveolar macrophage(PAM) by yeast two-hybrid method. [Result] There was no toxicity and self activation of bait protein in yeast hybridization system, and six proteins(FTH1, LGALS3, CORO1C, SNRPG, KRTAP5-3, ZNF598) interacting with N protein were indentified. It was confirmed that LGALS3 and SNRPG had specific interaction with N protein by return experiment and co-immunoprecipitation(CoIP) test. [Conclusion] The study lays a foundation for further studying the function of PEDV N protein and the pathogenic mechanism of PEDV.