Effective method of function analysis and screening of protein utilizing fluorescent light generated by cell-free protein synthesizing system

摘要

A method of detecting a reaction between a fluorescently labeled protein synthesized in a cell-free protein synthesizing system and a sample solution simply, in a short time and at high precision is provided. A case of detecting a binding reaction between an antibody fused with GFP and a sugar on the nanoparticle surface is explained. In a well of a microplate, a solution containing an antibody fused with GFP, and a solution containing a nanoparticle with a sugar reactive with the antibody fused with GFP adhered to a surface thereof are mixed to prepare a mixed solution A, and after the reaction, FCS measurement is performed.