THE INCREASE OF THE BIOLOGICAL ACTIVITY OF GLYCOPROTEIN PHARMACEUTICALS BY USING THE SIALIDASE INHIBITOR IN THE ANIMAL CELL CULTURE

摘要

The present invention by addition of the cyano acid inhibitor in the cell culture of protein (glycoprotein) per animal using a cell culture method relates to a method of producing a protein product per a high biological activity with high yield high titer. In the case of recombinant human erythropoietin compared to when they have not been used for inhibitors cyano acid content and biological activity was increased about 50%, the yield was confirmed to about 2 times or more increases.