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AFFINITY TRAP REACTOR AND SINGLE-STEP PROCESS FOR PURIFYING ANGIOSTATIN-LIKE FRAGMENT FROM HUMAN PLASMA USING THE SAME
AFFINITY TRAP REACTOR AND SINGLE-STEP PROCESS FOR PURIFYING ANGIOSTATIN-LIKE FRAGMENT FROM HUMAN PLASMA USING THE SAME
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机译:用相同的方法纯化人血浆中血管生成抑制素样片段的亲和捕集反应器和单步工艺
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摘要
It is intended to provide an affinity trap reactor allowing efficient progress of a reaction between an enzyme bonded to a support and a substrate without restriction in use depending on the types of the enzyme and substrate employed. An affinity trap reactor comprising a support having an enzyme and a molecule specifically reacting with the substrate of the enzyme bonded thereto; and a process for obtaining BL-angiostatin in a single step from plasminogen contained in a biological sample which comprises applying the biological sample containing plasminogen to an affinity trap reactor comprising a support having bacillolysin MA and lysine bonded thereto and reacting at 0 to 50 °C in the presence of isopropyl alcohol under calcium ion-free conditions to thereby obtain BL-angiostatin.
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