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Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same
Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same
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机译:亲和捕集反应器和使用其从人血浆中纯化血管生成抑制素样片段的一步法
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摘要
The present invention provides an affinity trap reactor that enables the reaction between an enzyme bound to a support and substrate to proceed efficiently without its applications being restricted by the type of enzyme and substrate used. The present invention relates to an affinity trap reactor composed of a support bound with an enzyme and a molecule that specifically binds with a substrate of the enzyme, and a single-stage process for obtaining BL-angiostatin from plasminogen contained in a biological sample, wherein a biological sample containing plasminogen is applied to an affinity trap reactor composed of a support bound with bacillolysin MA and lysine, and reacted under conditions of a temperature of 0 to 50° C. in the presence of isopropyl alcohol but in the absence of calcium ions.
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